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In addition to staining protocols, we discuss considerations for choosing cell lines and optimal BODIPY 493/503 stains fat droplets in living cells We routinely use these channels to detect antibody-fluorochrome conjugates and for most
 Manuals & protocols How can I stain live cells with Invitrogen™ BODIPY™ 493/503 dye (Cat. No.D3922)?. View answer. Invitrogen™ BODIPY™ 493/503 dye
 1 Jun 2016 Lab Protocol Title. Current Protocols in Cell Biology Red fatty acid staining is shown in red, lipid staining by BODIPY 493/503 is shown in green In the merged image, BODIPY 493/503 is shown as red, perilipin 2 shown as
 Results 1 - 10 of 18 Histochemical Detection of Lipid Droplets in Cultured Cells. Author(s): 3.1 Staining of LDs in Fixed Cells 3.1.1 BODIPY 493/503 1. Fix cells
 2 Aug 2011 Here, we describe the utilization of the BODIPY (493/503) dye in skeletal . The first half of the serial sections were stained with BODIPY as described above. Using the BODIPY (493/503), we were able to detect visible LDs .. of lipid droplets and associated proteins,” Current Protocols in Cell Biology,
 5 Sep 2016 Abstract. Lipid droplets (LDs) are ubiquitous, dynamic organelles and function as a storage depot for neutral lipids, including triglycerides and
 12 Nov 2009 protocol to induce adipocyte differentiation. Briefly, cells were formalin and stained with 0.01 mg/mL BODIPY 493/503 for 15 min (Invitrogen). combinations for probe generation were used for detection and. Zhang et al.
 Protocols for the detection of lipid droplets with nile red and BODIPY 493/503 are .. BODIPY 493/503 staining of lipid droplets is easily detected as green
 30 Oct 2013 Bodipy-493/503, goat anti-rabbit IgG conjugated to Alexa Fluor 568, goat . (D) The detection of the emitted fluorescence from the Bodipy or ORO stained . Test of freezing and thawing during the Bodipy staining protocol,
 Autofluorescence and LysoTracker red in vital worms were detected using filter . A BODIPY 493/503-based protocol stains major fat stores in C. elegans and
     
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