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Affinity purification with immobilized antigen. Covalently immobilizing purified antigen (i.e., the peptide or hapten used as the immunogen to induce production of antibody by the host animal) to an affinity support allows the specific antibody to be purified from crude samples.
 Affinity Purification of Antibodies. Day 1 : Coupling of ligands to beads. Ligand amounts for coupling: protein 5-10 mg per ml beads peptide 1- 5 mg per ml beads.
 15 Dec 2008 Abstract. This protocol describes purification of a polyclonal antibody that might be needed for immuno fluorescens microscopy or co-immuno
 Specific, purified antibodies can be Protocols, Methods in Molecular Biology, Vol.
 
 The following is a general protocol for affinity purification of antibodies from serum. Peptides or proteins are covalently bound to agarose beads by their free
 Conduct ELISA analysis with the corresponding antigen to confirm the recovered antibody and the removal of all the antibodies from the original serum. Concentrate the antibody to ~1-2mg/ml and dialyze against PBS with 0.02% sodium azide. Purified antibody can be stored refrigerated or aliquoted and frozen.
 Affinity Purification of Antibodies. Using Proteins Bound to Nitrocellulose by Michael Koelle, adapted from Will Talbot & Frank Solomon's lab, 1/98. 1.
 Affinity purification of antibodies. Additional Affinity purification (adapted form de Lange lab protocol). 1. Wash affinity the affinity column with 30 ml of PBS. 3.
 12 Jul 2007 A protocol for the purification of polyclonal antibodies from ovine serum Clarified serum is loaded directly onto the affinity column without prior
     
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