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In reaction #1 of glycolysis, hexokinase catalyzes transfer of phosphate to glucose from ATP, forming G6P. It is an example of an energy-coupled reaction and the Delta G zero prime is strongly negative, thanks to the ATP hydrolysis.
 ATP structure, ATP hydrolysis to ADP, and reaction coupling. The example above shows how ATP hydrolysis can be coupled to a biosynthetic reaction. . When there's a lot of ATP, though, phosphofructokinase (and other enzymes like it)
 spontaneous hydrolysis of ATP is coupled to the otherwise unspontaneous glucose phosphorylation. This reaction For example, the reaction considered on the previous slide is catalyzed by hexokinase. The role of the enzyme is to speed up
 13 Aug 2015
 The closing of the cleft in hexokinase is a striking example of the role of induced fit in enzyme action (Section 8.3.2). . How are these reactions coupled?
 This first reaction is endothermic and thus requires energy from a coupled reaction with ATP. ATP is used by being hydrolyzed to ADP and phosphate giving off energy and the phosphate for reaction with the glucose for a net loss of ATP in the overall glycolysis pathway.
 
 The first step in glycolysis is a priming reaction, where a phosphate group is added to However, the hexokinase reaction is highly regulated, with G6P providing a . For example, class I aldolase A is expressed in muscle, aldolase B in liver, Deficiencies in TIM are associated with haemolytic anaemia coupled with a
 This reaction requires energy and so it is coupled to the hydrolysis of ATP to ADP and Pi. • Enzyme: hexokinase. It has a low Km for glucose; thus, once glucose
 2 Oct 2000 Coupled reactions Are all the chemical transformations in these biosynthetic paths, for example, exergonic, so that the direction of .. of the two reactions written above it, rather, it IS the reaction catalyzed by hexokinase.
     
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