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 under 510 nm excitation was recorded using a microplate reader as described in (42). B. Cells were subject to the same loading protocol as was used for cytosolic A549WT cells in 24 well plates were loaded with 1 ?M MitoSOX (37°C, the antioxidant and 75 ?M CDDP for 24 hours before FACS analysis as in Figure 1B.
 15 Dec 2013 Has somebody done mitoSOX assay in 96 well plate using plate reader? I am wondering whether washing step in the protocol wont affect my cells Does anyone has experience with this analysis with suspension cells?
 30 Apr 2009 MitoSOX™ Red mitochondrial superoxide indicator, . The densitometric analysis was done using Scion Image and the data was presented The absorbance was recorded using a SpectraMax M5 plate reader (Molecular Devices, Inc.). according to the protocol provided by the assay kit manufacturer.
 27 Nov 2012 Here, we show new optimized fluorescence spectroscopy protocol that allows rapid Specificity of the plate reader-based superoxide measurements was confirmed by We show that limitations of the HPLC-based analysis can be conjugate (MitoSOX) has a specific excitation peak at 396 nm which can
 24 Apr 2014 plate reader-based fluorescence measurements protocol for NADPH oxidases (Nox) inhibitors. . HPLC analysis was performed using an Kalyanaraman, B. (2010) Hydroethidine- and MitoSOX-derived red fluorescence.
 MitoSOX Red reagent is a novel fluorogenic dye specifically targeted to mitochondria in live cells. Oxidation of MitoSOX Red reagent by superoxide produces red fluorescence. Readily Manuals & protocols Certificates of analysis (COA).
 use (for more detailed analysis, refer to the following: Dikalov &. Harrison Interestingly, it has been shown that the superoxide-specific product of MitoSOX Red . a microplate reader set to 535 nm excitation (Ex bandwidth: 25 nm) and. 635 nm Procedure. The following protocol has been optimized for 24-well plates.
 2) Measurement of mitochondrial superoxide by MitoSOX; 3) Measurement of We recommend scheduling a meeting prior to plate reader based analysis in order to determine the best protocol for sample preparation. This service may require
 Results 1 - 10 of 20 2.7 Measurement of “Mitochondrial Matrix” Superoxide Production in Human Fibroblasts Using MitoSOX Red 1. Multiwell plate reader. 2.
      
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