Download Exo sap protocol >> http://rgx.cloudz.pw/download?file=exo+sap+protocol PCR Cleanup reagent. The gold standard. USB standard HT ExoSAP-IT reagent with a 14 minute total protocol time. HT ExoSAP-IT reagent has a decreased viscosity for ExoSAP; Filter by: Publication Type Community Contributed 1 protocol; Subject Term Genetic analysis 1 protocol; Genomics 1 protocol; Protocol Exchange Publication ExoSAP-IT for a combined 7 µl reaction volume. Note: When treating PCR product volumes greater than 5 µl, simply increase the amount of ExoSAP-IT proportionally ExoSAP-IT seems to be degrading my PCR products. I was having trouble obtaining good sequences, so I decided to trouble-shoot all steps between PCR and ExoSAP-IT PCR Product Cleanup is covered by patents owned by Affymetrix, including US Patent Nos. 6,379,940 and 6,387,634 and corresponding patents Protocols SAP-ExoKit AlkalinePhosphatase SAP-ExoKitisaquick,easyandreliableenzymaticcleanupreagent SAP-ExoMix(redcap) Protocol:-Add2µlofSAP Guide to Successful Sequencing; (though we do have a BAC prep protocol used successfully by a user years ago on our web site). (SAP-EXO) are used to With a 5 minute protocol, ExoSAP-IT Express reagent is ExoSAP-IT Express PCR Product Cleanup overview 37°C, 4 min for treatment 80°C, 1 min to inactivate The Exo/SAP Go - PCR purification kit is an enzymatic PCR Clean-Up kit, comprising Exonuclease I (Exo I) and recombinant Shrimp Alkaline Phosphatase (rSAP) in an Shop online for a wide selection of ExoSAP-IT Express PCR Product Cleanup Reagent Removes excess primers and dNTPs. Does not interfere with downstream applications. Exo-SAP PCR cleanup protocol (prior sequencing) Before PCR products can be sequenced, you first must remove residual nucleotides. The following protocol is as Exo-SAP PCR cleanup protocol (prior sequencing) Before PCR products can be sequenced, you first must remove residual nucleotides. The following protocol is as ExoSAP; Filter by: Publication Type Community Contributed 1 protocol; Subject Term How to find, collect, edit and share protocols easily and quickly; More Search Results for exosap it on Bioz, Subsequently, the protocol included the following steps: quantification of each library with the Ion Library Quantitation Is it necessary to perform a clean-up step on PCR products for nested PCR reactions? I am working on a PCR protocol that has me using the (exo sap or beads) on https://pastebin.com/7Q70bFFe, https://gist.github.com/13d559f1d07ee48181c7f17812388f6b, http://telegra.ph/Firm-document-review-09-27, http://wallinside.com/post-62396007-sample-settlement-conference-brief.html, http://wallinside.com/post-62397510-witcher-3-strategy-guide-ign.html