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Results 1 - 10 of 11 Summary: (Life Sciences International, Basing-stoke, UK). 3 Methods 3.1 Loading Cells with Fluo-3/AM and Calcium Green-1/AM In general
 Fluo-3 is a long wavelength calcium probe having its absorption maximum at Fluo-3 AM is an acetoxymethyl ester derivative of Fluo-3 that can be easily loaded into . Protocol: 1. Add 16.5 mg Pluronic F127 to Fluo 3-AM/DMSO solution.
 Fluo 3 is also useful for caged calcium and others that are cleaved by the photoirradiation in the UV region. Fluo 3-AM is an acetoxymethyl ester derivative of
 The beginning of life, the act of fertilization, is regulated by Ca2+. Numerous Fluo-3 and Fluo-4 have also been extensively used for flow cytometry and Following is our recommended protocol for loading Cal-520™ AM esters into live cells.
 Fluo-3 has been used to image the spatial dynamics of Ca2+ signaling, in flow Invitrogen™ Related applications: Cell Viability, Proliferation & Function
 The Ca2+ indicator fluo-3 (Figure 1) was developed by Tsien and colleagues for use with visible-light F1242, Fluo-3, AM, Calcium Indicator, 20 x 50 µg.
 in Ca2+ signaling.2-4 Fluo-3 has also been extensively used for flow cytometry;5 .. with fluo-3 AM and fluo-4 AM according to protocols supplied by Molecular.
 Fluo-3 has been used to image the spatial dynamics of Ca2+ signaling, in flow Invitrogen™ Related applications: Cell Viability, Proliferation & Function
 Protocol: General Protocol (for Human T cells) Reagents: 2mM Fluo 3/AM/DMSO (1mg Fluo 3/AM in 442?l DMSO) Pluronic F127 Hanks-balanced salt solution
 This protocol uses Fluo-3 AM, in combination with flow cytometry, to monitor T cell receptor-triggered calcium changes over time.
     
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