Creative Biostructure is highly-experienced in custom mutagenesis libraries construction services for directed evolution. Site-saturation mutagenesis (SSM) can generate random or targeted mutations and screen the library of protein variants for functional alterations. Notably, our protein evolution and engineering platform holds many advantages for custom SSM libraries services, including extremely cost-efficient, fast delivery time, fewer variants to screen and, systematic identification of beneficial amino acid substitutions.

Site-saturation mutagenesis (SSM) is a simple, fast and efficient technique using PCR amplication with degenerate synthetic oligonucleotides as primers. Since a specific amino acid residue within the protein sequence can be identified to be critical for the protein’s function, it is very important to determine the ideal amino acid residue for this position. SSM is a form of random mutagenesis, allowing the substitution of specific sites against all 20 possible amino acids at once. It is well-known as sequential permutation, SSM used by Creative Biostructure in combination with the powerful screeing assay is the most systematic mutagenesis strategy to identify amino acid substitutions that fulfill customers’ protein engineering projects.

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