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1 Jun 2012 This is the first report of endophytic bacteria from the leaves of P. vulgaris Each isolation procedure was done in triplicate for each cultivar.
27 May 2016 To date, plant endophytic bacteria have mainly been studied in roots of crop such as dehydration, freezing, ionizing radiation, UV light and elevated .. chloroform isolation protocol (Chomczynski and Sacchi, 2006) was utilized for detection of microbial cellulases on agar plates using Gram's iodine.
endophytic fungi: Isolation, Purification, Characterization and Bioassays”, submitted by Mr. Ashutosh Kumar, .. iodine solution. Saha et al., developed a rapid staining method of these endophytic fungi using rose bengal stains. 54 .. Concomitant administration of radiation therapy or chemotherapeutic agents such as L-.
28 Apr 2014 these two lineages in an extraordinary example of coevolutionary radiation. We began our study of the mycosome cycle by isolating endophytic fungi from . vector (Invitrogen), and transformed following manufacturer's protocols. MR is iodine-based, and stains fungal cytoplasm yellow, short chain
Table 3.4 Summary of isolation of endophytic fungi from different plants. 81 . However, there is no certainty that any surface sterilization method will kill all mostly blue reaction to the apical apparatus of the asci in iodine reagents .. transcribed spacer region and intergenic spacer of the nuclear rRNA repeat unit evolve.
involves chemical investigation of fungicolous and endophytic fungi that resulted in The fungal secondary metabolites isolated during the course of these studies Mosher's method, X-ray crystallography, and/or by ECD spectral analysis in combination exposure to UV light at 254 nm or by exposure to iodine vapor.
20 May 2015 Organism isolation is the first critical step in this cultivation procedure. A common endophyte isolation protocol consists of a surface sterilization
the best opportunities to isolate endophytic microorganisms as well as production of imprint method (Schulz et al.,1993), also sample washed distilled water was . After incubation period, culture plates were treated with iodine, observing .. Nuclear magnetic resonance is a phenomenon which occurs when the nuclei of.
22 Dec 2015 Endophytic bacterial population was isolated from Spartina maritima tissues, a heavy metal . The protocol of surface-sterilized was as follows: first surface washing under using an agarose gel (1.5%) and revealed by UV radiation. by flooding the plates with iodine–potassium iodide solution (lugol).
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