Champions League clash with AS Roma on 22 November, when Los Colchoneros secured a 2-0 victory to keep their hopes of qualifying for the knockout stages of the competition alive.
The Spaniard missed the subsequent three wins over Levante, Elche and Real Sociedad and will remain on the sidelines for the key clash with Chelsea.
Juanfran's absence is an important blow for Simeone as the 32-year-old right-back would surely have make the starting line-up at Stamford Bridge if he had been fit.
Simeone has recovered his other specialist for that position with Vrsaljko returning to the squad.
