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MUSCLE User Guide. Multiple sequence comparison by log-expectation. by Robert C. Edgar. Version 3.5. August 2004. /muscle. email: muscle (at) drive5.com.
If muscle completes successfully, it will create a file seqs.afa containing the alignment. By default, output is created in "aligned FASTA" format (hence the .afa
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The quick reference evidence informed Muscle Manual is designed as a quick reference guide for health care students, therapist, practitioners, doctors & instructors, and as a companion to the Physical Assessment Manual, Orthopedic Conditions Manual and Physical Medicine Manual.
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