The Glutathione S-transferase (GST) fusion system is a comprehensive system to express, purify, and detect GST-tagged proteins. The GST affinity tag allows protein purification under mild conditions and easy cleavage, keeping the proteins in their native structure and function.Three major parts comprise this system: pGEX plasmid expression vectors, GST-tagged protein purification, and GST-tagged protein detection.
Advantage of GST fusion system:
1. All pGEX vectors provide a tac promoter to express proteins at high-level and with chemical induction;
2. Purify active proteins under mild and nondenaturing conditions;
3. Suitable for one-step, high-yield affinity chromatography purification based on glutathione sepharose media;
4. Easy cleavage of target protein from fusion protein with multiple proteases such as Thrombin or Factor Xa;
5. Convenient detection of GST fusion protein with antibodies.
https://www.creative-biostructure.com/gst-tagged-proteins-552.htm
