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14 Feb 2015 In the KeratinoSens™ protocol, Nrf2-dependent luciferase induction and the A single chemical showed divergent results and analysis by four
KeratinoSens™ assay protocol Define a detailed and exact protocol Proposed by BASF, based on 54 chemicals; Recent analysis on 103 chemicals with
KeratinoSens™ Test. The KeratinoSens™ test is an in-vitro method on human keratinocyte Post incubation, analysis by HPLC-UV. ? Considered positive if
INVITTOX Protocol ARE-reporter cell line KeratinoSens to identify potential skin sensitizing chemicals . DATA ANALYSIS/ PREDICTION MODEL. Chemicals
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22 Oct 2014 Global analysis yields a regression of in vitro data to LLNA pEC3 with an R2 of 60% The KeratinoSens™ assay measures Nrf2 induction in a . As the assay used here is not the validated DPRA protocol, it is of relevance
KeratinoSens™ skin sensitization assay protocol. Cell Line, KeratinoSens™ cell line. Analysis, Induction of luciferase reporter gene expression and cell viability.
2012 DPRA, MUSST, hCLAT, and Keratinosens evaluated as part of an integrated testing strategy for in measuring chemical induced antigen-specific production of cytokines by FACS analysis (10). Protocol for epiCS SSPT test method.
The LuSens provides the same readout as KeratinoSens™; both can be used . reagent and according to manufacturer's protocol (Polyplus-transfection SA, USA). Briefly, for luminescence analysis, 200 ?L of cell suspension corresponding
16 May 2014 Test chemicals are considered positive in the KeratinoSens™ if they induce a .. analysis using a lower dilution factor (e.g., 1.33 or v2 (=1.41) fold dilution . DB-ALM (INVITTOX) (2013) Protocol 155: KeratinoSensTM., 17pp.
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